Troubleshooting Eosin Staining, and a Brighter Eosin Stain

Hirschsprung's disease diagnosis: Comparison of immunohistochemical, hematoxilin and eosin staining

BACKGROUND The diagnosis of Hirschsprung's disease (HD) is based on the absence of ganglion cells. In hemotoxilin and eosin (H and E) as well as acetylcholine esterase staining there are limitations in the diagnosis of immature ganglion cells in neonates. METHODS In this prospective study, 54 biopsies taken from suspected HD patients (five mucosal specimens and 49 full thickness specimens) we...

A modified haematoxylin and eosin stain for histologicl sections of lymph nodes.

The Staining Act: An Investigation into the Nature of Methylenblue-Eosin Staining.

The various theories of the nature of the act of staining of animal cells, tissues and products, which have been put forward by different writers fall into three groups: (i) that the process of staining is a chemical reaction between the dye on the one hand, and the cell or tissue elements on the other; (2) that in staining the dye is adsorbed by the tissue elements stained; and (3) that the dy...

Further Studies on Eosin Hemolysis

Additional experimental work on the subject of eosin hemolysis has been carried out. This indicates that red cells may be protected against the toxic action of eosin in sunlight by the presence of inorganic reducing agents. It is pointed out that a marked parallelism exists between the substances which react with the Folin and Denis reagent and the compounds which afford protection to red cells...

Comparison of Special Stains for Keratin with Routine Hematoxylin and Eosin Stain

BACKGROUND Keratins are the most abundant proteins and are characteristic findings in many epithelial pathologies, making it a diagnostically important marker, both histopathologically and immunohistochemically. Since, immunohistochemistry is an expensive diagnostic tool, special stains to detect the degree of keratinization could serve as a faster and economic option. The aim of the present st...